Figure 1.

Schematic diagram of hybrid SELEX method for selection of MGB2 and MGB1-specific DNA aptamers. Hybrid SELEX is divided into (A) a protein-based SELEX in which GST-MGB2 conjugated beads (brown) and GST-MGB1 proteins conjugated beads (purple) were used. The protein SELEX was done for 21 rounds for MGB2 and 4 round for MGB1. Subsequently, the enriched pools of both proteins were transferred to cells (B) to start cell-based SELEX in which HCAEC and MCF10A were used as counter selection cell lines. MCF7 was used as the target selection cell line for MGB2 and MDA-MB-415 was used as the target selection cell line for MGB1. After 7 rounds of cell-SELEX to each target cell line, the final pools for both targets were transferred back to the proteins for another 2 additional rounds for each MGB2 and MGB1 to make the library more specific to MGB2 and MGB1.