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. 2017 Nov 3;7:14487. doi: 10.1038/s41598-017-13751-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Binding of MAMB1 and MAMA2 aptamers to their breast cancer cells in whole Blood lysate by flow cytometry. (A–C) Mean of fluorescence intensity of MDA-MB-415 cancer cells and PBMC normal cells at 200 nM (A), 100 nM (B) and 50 nM (C) concentrations of MAMA2 aptamer. (D–F) Mean of fluorescence intensity of MCF7 cancer cells and PBMC normal cells at 200nM (D), 100nM (E) and 50nM (F) concentrations of MAMB1 aptamer. Two cell numbers from both cancer and normal cells were used: 10⁴ and 10³ cells. Values are shown as means ± S.E.M. of three trials. The statistical significance was determined by one way ANOVA followed by Fisher LSD multiple tests using SPSS software (SPSS, version 23). P Values less than 0.05 were considered to be significantly different.