Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 3;7:14487. doi: 10.1038/s41598-017-13751-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Binding curves of 6-FAM-labeled aptamers to MCF7 and MDA-MB-415 target cell lines. (A) MAMB1 K_d curve: MCF7 cells were incubated with increasing concentrations (nM) of MAMB1 then was evaluated by flow cytometry. (B) The predicted secondary structure of MAMB1 aptamer using RNAstructure software. (C) MAMA2 K_d curve: MDA-MB-415 cells were incubated with increasing concentrations (nM) of MAMA2 then was evaluated by flow cytometry. (D) The predicted secondary structure of MAMA2 aptamer using RNAstructure software. To calculate the apparent K_d of the interaction of MAMB1 and MAMA2 to their corresponding cell lines, the mean fluorescence intensity of the aptamer-cell dissociation vs. concentration was fit to the equation Y=Bmax X/(K_d+X). R0 ssDNA library was used as control. The Kd graphs are the average of three trials. Values are shown as means ± S.E.M.