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. 2017 Nov 3;7:14982. doi: 10.1038/s41598-017-14808-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of gene expression in pleural macrophages with and without antibiotics treatment in vitro. Pleural macrophages were isolated from five patients before antibiotics treatment and cultured in vitro for 36 hours with or without antibiotics. Cells were collected for RNA isolation and real-time PCR to quantify the transcript level of the indicted genes. Transcript level of each gene was normalized to GAPDH (ct). The ratio of ct with and without antibiotics is shown. Error bar, SEM; *P < 0.05; **P < 0.01.