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. 2017 Nov 3;7:14481. doi: 10.1038/s41598-017-14976-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MRI assisted RFA in orthotopic liver tumor model. (A) Coronal T2 weighted MRI of tumor model showing liver tumor in encircled region (B) Intratumoral injection of 300 µL of 10 mg/ml nCP:Fe. T2 weighted axial MRI (C) before and (D) after intratumoral sample injection. Tumor region marked within yellow circle. The dark region within the tumor (arrow mark) is due to T2 contrast of nCP:Fe. (E) RFA of the liver tumor. Probe was inserted to tumor (at intersection of injected and non-injected area), 10 W power was applied for 1 minute (F) Axial MRI of the liver showing the bright ablated region (G) Photograph of the rat liver showing ablated tumor within circle (H) TTC stained liver section showing sample treated necrotic tumor (unstained: Area 1) and untreated control tumor showing viability (stained pink: Area 2).