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. 2017 Nov 3;7:14493. doi: 10.1038/s41598-017-15141-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-27a targets 3′-UTR of mouse Fasn and Scd1 mRNA. (a,b) Sequences alignment of mouse (mmu) miR-27a and miR-27b with the 3′-UTRs of Fasn mRNA of mouse (Mmu), human (Hsa), rat (Rno), chimpanzee (Ptr) and dog (Cfa) (a) and of Scd1 mRNA of mouse (b). The seed regions of miR-27a and miR27b are indicated in bold. (c,d) Luciferase activity assays of luciferase reporter conjugated with 3′-UTR or 3′-UTR mutant (without predicted miR-27a binding site) of Fasn (c) and Scd1 (d) in HEK293T cells. (e–h) Real-time PCR analysis of miR-27a, Fasn, Scd1 and Accca in mouse primary hepatocytes transfected with miR-27a mimics (e,f) or miR-27a inhibitor (g,h). All data are shown as mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired two-tailed student’s t-test.