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. 2017 Nov 3;8:1307. doi: 10.1038/s41467-017-01503-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Dual-color SIM images of lysosomes and mitochondria in live cells. a, b, d, e, f Representative time-lapse SIM images reveal different types of dynamic physical interactions between lysosomes and mitochondria in live U2OS cells stained with Lysosome-565 (green) and Atto 647N (magenta) (more frames are shown in Supplementary Movies 3–5). c A SIM image of a whole live U2OS cell. g Cross-sectional profile of the tubular structure of the mitochondrion in f at a full-width at the half-maximum (FWHM) of 91 nm. Each SIM frame was acquired over 270 ms (i.e., a raw data exposure time of 30 ms). For the time-lapse images, the time interval between each dual-color SIM image was set to 6 s. Scale bars, a, b, d, e, f 1 μm, and c 4 μm