Fig. 1.

The interaction of CBC with ARS2 is enhanced in the presence of m⁷GTP. a Schematic diagram of the domain structure of ARS2 based on ref. ²⁶. b Gel filtration chromatogram and Coomassie-stained SDS-PAGE of CBC-ARS2 in the presence and absence of m⁷GTP. Purified recombinant CBC and ARS2^147–871 were mixed with (orange) or without (blue) 500 µM m⁷GTP and subjected to gel filtration. Their elution profiles were overlaid (left) and single fractions analysed by SDS-PAGE (middle and right). Purified recombinant CBC and C-terminally truncated ARS2^147–845 were mixed with 500 µM m⁷GTP and analysed in a similar way (bottom left). c ITC data and curve fit to derive the affinity of different ARS2 constructs for CBC. CBC or m⁷GTP-CBC in the sample cell was titrated by ARS2. In each panel, the upper graph shows the raw data and the lower graph shows the ligand concentration dependence of the heat released upon binding after normalisation. K _D values and standard deviation represent the average from at least two independent experiments. d Sequence alignment of the C-terminus of ARS2. Conserved residues are highlighted in red. Figure made with ESPript⁵⁴