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. 2017 Nov 3;7:14979. doi: 10.1038/s41598-017-15135-9

Table 1.

List of primers used for site-directed mutagenesis.

Mutant plasmids Primers used for site-directed mutagenesis
ERK3 L290P 5′-aatttgttccaggaaatccggtgcttctcgactaattcc-3′
ERK3 L290V 5′-tccaggaaatccactgcttctcgactaattcctgg-3′
ERK3 S189A 5′-cccataagggtcatcttgctgaaggattggttac-3′
ERK3 kinase dead (KD) 5′-ggggatcagtaaggacaattgccgcgatggctactcttttgtcac-3′