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. 2017 Nov 3;7:15033. doi: 10.1038/s41598-017-15153-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GFP reporter expression driven by BAC16. Embryos of Xenopus laevis at neural plate (A–D: dorsal view, anterior to the left) and tailbud stages (A’–D’, A”,B”: lateral view, anterior to the left) after injection with BAC16 reporter constructs at the one-cell stage. A–A”, B–B”: GFP expression after injection of full length BAC16 reporter (BAC16-FL) in living Xenopus embryos (A–A”) or as revealed by GFP in situ hybridization (B–B”). C,C’: Absence of GFP expression domains after injection of BAC16-P2D3 deletion construct. D,D’: GFP expression domains after injection of BAC16-P2D4 deletion construct recapitulate those observed after BAC16-FL injection. Insert in D’ shows somitic expression in trunk region of a different embryo (tail end to the right). Abbreviations: epi: epidermis; le: lens placode; ll: lateral line placodes; np: neural plate; olf: olfactory placode; ot: otic placode/vesicle; pp: pharyngeal pouches; PPR: preplacodal region; som: somites.