Table 1.
Expression domains mediated by BAC15.
| Expression Constructs | Neural plate stage embryos (14–18) | Tailbud stage embryos (23–40) | |||||
|---|---|---|---|---|---|---|---|
| Epidermis % (n) | PPE % (n) | Neural plate % (n) | Epidermis % (n) | Head ectoderm/ placodes % (n) | Somites % (n) | Pharyngeal pouches/gut % (n) | |
| BAC15-control1 | 0 (53) | 0 (53) | 0 (53) | 0 (8) | 0 (8) | 0 (8) | 0 (8) |
| BAC15-FL2 | 81 (124) | 75 (124) | 81 (124) | 74 (114) | 74 (114) | 71 (114) | 30 (114) |
| BAC15-P1D13 | nd | nd | nd | nd | nd | nd | nd |
| BAC15-P1D23 | 78 (49) | 73 (49) | 78 (49) | 76 (129) | 62 (129) | 75 (129) | 33 (129) |
| BAC15-P1D33 | nd | nd | nd | nd | nd | nd | nd |
| BAC15-P1D43 | 79 (43) | 72 (43) | 79 (43) | 90 (10) | 90 (10) | 90 (10) | 60 (10) |
| BAC15-P2D13 | 0 (46) | 0 (46) | 0 (46) | 0 (64) | 0 (64) | 3 (64) | 0 (64) |
| BAC15-P2D23 | 0 (44) | 0 (44) | 0 (44) | 0 (61) | 0 (61) | 0 (61) | 0 (61) |
| BAC15-P2D33 | 97 (37) | 97 (37) | 89 (37) | 50 (4) | 50 (4) | 25 (4) | 0 (4) |
| BAC15-P2D43 | 100 (29) | 97 (29) | 93 (29) | 67 (6) | 67 (6) | 50 (6) | 0 (6) |
n: number of embryos analyzed; nd: not determined.
1Empty BAC15 (no insert).
2BAC15 full length (FL).
3BAC15 deletion constructs (see Fig. 3).