Table 2.
Expression domains mediated by BAC16.
| Expression Constructs | Neural plate stage embryos (14–18) | Tailbud stage embryos (23–40) | |||||
|---|---|---|---|---|---|---|---|
| Epidermis % (n) | PPE % (n) | Neural plate % (n) | Epidermis % (n) | Head ectoderm/ placodes % (n) | Somites % (n) | Pharyngeal pouches % (n) | |
| BAC16-control1 | 0 (59) | 0 (59) | 0 (59) | 0 (8) | 0 (8) | 0 (8) | 0 (8) |
| BAC16-FL2 | 71 (234) | 67 (234) | 68 (234) | 60 (148) | 76 (148) | 35 (148) | 0 (50) |
| BAC16-P1D13 | 85 (54) | 83 (54) | 78 (54) | 78 (124) | 69 (124) | 23 (124) | 0 (82) |
| BAC16-P1D23 | nd | nd | nd | nd | nd | nd | nd |
| BAC16-P1D33 | 55 (29) | 41 (29) | 41 (29) | 65 (69) | 46 (69) | 26 (69) | 0 (69) |
| BAC16-P1D43 | 72 (152) | 68 (152) | 71 (152) | 73 (146) | 60 (146) | 50 (146) | 0 (146) |
| BAC16-P2D13 | 7 (41) | 0 (41) | 0 (41) | 0 (51) | 0 (51) | 2 (51) | 0 (51) |
| BAC16-P2D23 | nd | nd | nd | nd | nd | nd | nd |
| BAC16-P2D33 | 0 (48) | 0 (48) | 0 (48) | 0 (73) | 0 (73) | 0 (73) | 0 (73) |
| BAC16-P2D43 | 69 (36) | 50 (36) | 44 (36) | 61 (70) | 49 (70) | 33 (70) | 16 (70) |
n: number of embryos analyzed; nd: not determined.
1Empty BAC16 (no insert).
2BAC16 full length (FL).
3BAC16 deletion constructs (see Fig. 3).