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. 2017 Oct 30;8:841. doi: 10.3389/fphys.2017.00841

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Copyright © 2017 Fung, Wang, Nyegaard, Heit, Fairn and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SR-BI is required for HDL internalization. (A) The addition of unlabeled anti-SR-BI nanobody which binds to the extracellular domain of SR-BI partially blocks HDL internalization. n = 3; approximately 40 cells were observed for each condition. (B) HCCMECs were co-transfected (molar ratio 1:100) with GFP and siRNA against SR-BI or a non-targeting control (Ctrl) to test the effect of SR-BI on HDL internalization. (C) 48 h post transfection, SR-BI siRNA transfected cells showed a decrease in HDL internalization. n = 5; approximately 25 transfected cells were observed for each condition. Scale Bars = 15 μm. ^*p < 0.01. (D) Effective knockdown of SR-BI in co-transfected cells was verified by immunofluorescence (SR-BI in green; mCherry in red).