Figure 6.

PDZK1 is not required for HDL internalization in HCCMECs. (A) Western blot showing the expression of endogenous PDZK1 in HCCMECs compared to positive control, HCCMECs overexpressing PDZK1. (B) 48 h post transfection of GFP alone or GFP-PDZK1, DiI-HDL was internalized for 10 min which showed that over-expressed GFP-PDZK1 had no significant effect on DiI-HDL internalization. Quantification can be found in (C); n = 5; approximately 25 transfected cells were observed for each condition. Scale Bars = 15 μm.