Figure 3.

Analysis of Tas2r143-reporter mice in the lower respiratory tract. (A) Immunofluorescence staining of cryosections in the trachea, bronchus and bronchiole. EGFP-positive epithelial cells prominently colocalized with the tuft cell marker DCLK1. Nuclei were counterstained with DAPI. Squares indicate enlarged areas. Scale bars: 50 μm. (B–E) RNA-seq analysis of gene expression levels in purified EGFP-positive cells compared with Tomato-positive cells. The relative expression was calculated as log₂(EGFP/Tomato). EGFP- and Tomato-positive cells were isolated from the tracheas of the reporter mice (n = 3).