Figure 1.

Development of CD141⁺ and CD1c⁺ dendritic cell (DC) in hu mice. (A) Gating strategy used to define DC subsets in the bone marrow (BM) of hu mice. After gating on live singlet cells, DCs were defined as hu CD45⁺ HLA-DR⁺, CD19/20⁻, and CD1c⁺ or CD141^+. (B) Frequency of human leukocytes, CD141⁺ DC and CD1c⁺ DC engrafted in the organs of NSG-A2 mice expressed as % mononuclear cells (huCD45) or as % hu CD45⁺ cells (CD141⁺ and CD1c⁺ DC). (C) Frequency of human leukocytes, CD141⁺ DC and CD1c⁺ DC expressed as absolute cell numbers per organ. BM data are quantitated per tibia and femur, and blood per ml.