Fig. 4.

Combination of lapatinib and NVP-AEW541 – but not NVP-BEZ235 inhibits cell migration in HCC38CisR measured by scratch assay. a Microscopic images were obtained before (0 h) and 24 h after applying a pipet tip-induced scratch in a nearly confluent cell monolayer. Data shown are one typical experiment out of three independent experiments. b Average migration, estimated as space occupied after 24 h, showed that only the combination of 1.5 μM NVP-AEW541 and 1 μM lapatinib significantly reduced cell migration (**p < 0.01). c Cell proliferation assays applied under the conditions of (b) (24 h incubation). Only 20 nM NVP-BEZ235 significantly reduced cellular proliferation (n = 3, *p < 0.05)