Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 3;12:7. doi: 10.1186/s13029-017-0067-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — Illustration of the features of FLIM-FRET analyzer. a Multistep menu for single cell segmentation (step 1), whole image lifetime calculation (step 2), average lifetime single cell calculation (step 3), and batched multi FLIM image experiments (step 4). b Fluorescence-based (white) single cell segmentations (red). c Lifetime map. d Instrument response function (blue), Lifetime raw data (red), Lifetime fitting (green). e Average lifetime calculation at the single cell level. f Batched analysis of multiple experiments and the average single cell FLIM measurement display