Fig. 8.

SiNPs inhibit activation of TRPV4 currents in perforated patch-clamp recordings. a, b Experiments in TRPV4-transfected HEK293T cells showing the effects of the TRPV4 agonist GSK1016790A in the absence (a) and in the presence (b) of SiNPs. The data points represent the amplitude of the currents measured at -75 and +75 mV. The colored data points correspond to the current traces displayed on the panels shown on the right. c Average increase in the amplitude of currents (recorded at -75 and +75 mV) induced by the application of 10 nM GSK1016790A during 2 min, in the absence (n = 5) and in the presence (n = 6) of 30 μg/ml SiNPs. The * and ** symbols indicate P < 0.05 and P < 0.01; unpaired t test