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. 2017 Nov 3;15:224. doi: 10.1186/s12967-017-1327-0

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — TALEN design and activity. a A schematic diagram of TALEN targeting exon 4 of the porcine P53 gene. b A schematic diagram of TALEN plasmids specific for P53 gene. c The detection of TALEN activity using a luciferase SSA recombination assay. Luciferase activity was increased by 55.35-fold compared to the control activity