Table 1.
Gene-specific primers used in SSH and semiquantitative RT-PCR analysis
| Gene | Primer Sequence (5′-3′)a | GenBank accession number | |
|---|---|---|---|
| ADAMTS1 | Fwd | CGATAAATGTGGCATCTGTGGAG | NM_001101080 |
| Rv | AGCCCACACGACTTGGAACACTC | ||
| ADAMTS9 | Fwd | GGAAATCCGTATTGGGAATGCTG | NM_001206573 |
| Rv | CATCTAGCCTGCTGTACTTGGC | ||
| ARAF | Fwd | TCTAACAACATCTTCCTGCACGAG | NM_001014964 |
| Rv | GGCAACTCATCAGCCTGGGTAC | ||
| CAPN2 | Fwd | TGAGGGCTTCGAGGACTTCACTG | NM_001103086 |
| Rv | GCTGTCACTGGTGAGTGTGTCAG | ||
| CRISPLD2 | Fwd | CTATGGGATCCTGGACGACAGG | AY369781 |
| Rv | CCTGCAAGTTCACTGCCTGACG | ||
| CYP19A1 | Fwd | GTCCGAAGTTGTGCCTATTGCCAGC | NM_174305 |
| Rv | CCTCCAGCCTGTCCAGATGCTTGG | ||
| FKBP4 | Fwd | TCCATTCCTCGTGAATGAATGTCC | NM_001034322 |
| Rv | TGAACCTTCAGCAACGTGCAGAG | ||
| FKBP5 | Fwd | ACTGGAGCAGGCTGCCATTGTC | XM_615814 |
| Rv | CTTGAACATGTTGGCATAGATTCTG | ||
| GAPDH | Fwd | TGTTCCAGTATGATTCCACCCACG | NM_001034034 |
| Rv | CTGTTGAAGTCGCAGGAGACAACC | ||
| KITLG1 | Fwd | AGTAACCGTGTGACTGATGATGTG | NM_174375 |
| Rv | AGAATGCTGGCAATGCTACGGCTG | ||
| KITLG2 | Fwd | AGTAACCGTGTGACTGATGATGTG | NM_174375 |
| Rv | CTAAGGGAGCTGGCTGCAACAG | ||
| L3MBTL3 | Fwd | GTTATTACAGATGAGAGTGAGATGG | AY437805 |
| Rv | ACTTAAAGGACAGAAAGACGGC | ||
| L3MBTL3 | Fwd | CTTACCTGGATGTGAAGAACATGG | AY437805 |
| (3’end of ORF) | Rv | CTGCAAGGTCTAAGACAGAGCTC | |
| MMP1 | Fwd | CTTGGACTTGCTCATTCTACTGAC | NM_174112 |
| Rv | GGCATCGATGCTCTTCACCGTTC | ||
| MRO | Fwd | GGTGAGTTCTGAAGTCATCCATG | BC146146 |
| Rv | CCTGGTGTGCCGACCACCTTC | ||
| NUDT10 | Fwd | GACAGGTGAGCTCTTTCACACTC | NM_001035488 |
| Rv | GGAGTTATGTCTAGAGGCACAGTC | ||
| NUDT11 | Fwd | GAACAGCAAAGATGTCCAGGATTG | NM_001101296 |
| Rv | CACACACATGGTGCCTGGAGAG | ||
| P4HA3 | Fwd | TTGGCAAGGTGGCCTATGACATG | NM_001001598 |
| Rv | CTGGAGCAGCAGGTAAGGACTG | ||
| POSTN | Fwd | CCAAAGCCCACTGCCAGTTCTC | AY445072 |
| Rv | GAAAGCCACTTTGATGTGAAGAATGAG | ||
| PSAP | Fwd | CTTCCTCCTGTACCCTCAGGAC | NM_174161 |
| Rv | GGGATGACGTTCTCTGAGACCAC | ||
| PTGS2 | Fwd | GCATTCTTTGCCCAGCACTTCACCC | NM_174445 |
| Rv | CTATCAGGATTAGCCTGCTTGTCTGG | ||
| RBP1 | Fwd | CGGTCGACTTTACCGGGTACTG | NM_001025343 |
| Rv | GTCATGTCACTCATTCCTAGAGAC | ||
| SDC4 | Fwd | TCCGAGAAACTGAGGTCATCGAC | XM_584869 |
| Rv | GGTACACCAGCAGCAGCACGAG | ||
| TIMP1 | Fwd | CGTCATCAGGGCCAAGTTCGTG | NM_174471 |
| Rv | GCAAGGACTGCCAGGTGCACAG | ||
| TIMP2 | Fwd | TGCAATGCAGACATAGTGATCAGG | NM_174472 |
| Rv | CGCTTCTCTTGATGCAGGCGAAG | ||
| TNC | Fwd | CTATGTGCCCATTGCAGGAGGTG | NM_001078026 |
| Rv | AACTTGGTGGTGATGGTTGAGCTC | ||
| USP53 | Fwd | GAGAGCATACCCACCAGTCAGATG | BC149007 |
| Rv | GTTACTTTGACATCAGGAGTAGAGTC |
Fwd foward primer, Rv Reverse primer
aAll primers were designed and validated by the authors