Table 2.
Genes found to be differentially expressed in bovine granulosa cells of OF compared to DF
| Gene | Accession Numbera |
Freqb | Accession Numberc | Identity (%)d |
E value |
Descriptione |
|---|---|---|---|---|---|---|
| ACTG1 | EG565281 | 3 | NM_001033618 | 100% | 0 | BT Actin gamma 1 |
| ADAMTS1 | GR508913 | 1 | NM_001101080 | 100% | 2e−126 | BT ADAM metallopeptidase with thrombospondin type 1 motif 1 |
| APP | EG565335 | 1 | NM_001076796 | 100% | e−107 | BT Amyloid beta precursor protein |
| ARAF | EG565327 | 4 | NM_001014964 | 100% | 0 | BT A-Raf proto-oncogene. Serine/threonine kinase |
| ASB9 | EG565323 | 2 | AY438595 | 99% | 4e−179 | BT Ankyrin repeat and SOCS box-containing 9 |
| BIRC2 | EG565293 | 1 | XM_015466434 | 100% | 2e−67 | BT Baculoviral inhibitor of apoptosis repeat containing 2 |
| CAPN2 | EG565332 | 1 | NM_001103086 | 100% | 8e−57 | BT Calpain 2, (m/II) large subunit |
| CAV1 | EG565338 | 20 | AY823915 | 100% | 0 | BT Caveolin 1 |
| CD83 | EG565265 | 1 | NM_001046590 | 99% | 0 | BT CD83 molecule |
| CDC42SE2 | EG565271 | 1 | NM_001102537 | 96% | 2e−180 | BT CDC42 small effector 2 |
| CRISPLD2 | EG565333 | 16 | AY369781 | 100% | 0 | BT Cysteine-rich secretory protein LCCL domain containing 2 |
| CSRP3 | EG565284 | 1 | NM_001024689 | 99% | 3e−165 | BT Cysteine and glycine-rich protein 3 |
| CTSK | EG565324 | 1 | NM_001034435 | 100% | 5e−168 | BT Cathepsin K |
| DHTKD1 | EG565315 | 1 | NM_001205838 | 100% | 3e−170 | BT Dehydrogenase E1 and transketolase domain containing 1 |
| EGR1 | EG565268 | 1 | AY924307 | 98% | 9e−127 | BT Early growth response 1 |
| EIF4E3 | EG565311 | 1 | NM_001102306 | 99% | e−179 | BT Eukaryotic translation initiation factor 4E family member 3 |
| FKBP5 | EG565325 | 6 | NM_001192862 | 100% | 0 | BT FK506-binding protein 5 |
| GEM | EG565288 | 2 | NM_001083732 | 100% | 0 | BT GTP binding protein overexpressed in skeletal muscle |
| GFPT2 | EG565318 | 5 | NM_001076883 | 99% | 4e−168 | BT Glutamine-fructose-6-phosphate transaminase 2 |
| GRIA3 | EG565320 | 3 | NM_001206059 | 99% | 2e−166 | BT Glutamate ionotropic receptor AMPA type subunit 3 |
| KIT | EG565345 | 1 | XM_005207937 | 99% | 7e−110 | BT KIT proto-oncogene receptor tyrosine kinase |
| KITLG | EG565276 | 5 | NC_007303 | 99% | 0 | BT KIT ligand |
| L3MBTL3 | EG565310 | 2 | AY437805 | 100% | 7e−73 | BT Histone methyl-lysine binding protein |
| LRIG1 | EG565303 | 1 | XM_002696962 | 100% | 2e−84 | BT Leucine-rich repeats and immunoglobulin-like domains 1 |
| MAOA | EG565344 | 4 | NM_181014 | 99% | 2e−109 | BT Monoamine oxidase A |
| MMP1 | GR508914 | 3 | NM_174112 | 100% | 8e−83 | BT Matrix metallopeptidase 1 |
| MORF4L1 | EG565343 | 1 | NM_001035448 | 98% | 4e−38 | BT Mortality factor 4 like 1 |
| MRO | EG565273 | 4 | XM_005224181 | 100% | 1e−95 | BT Maestro |
| NT5E | EG565299 | 1 | NM_174129 | 100% | 0 | BT 5′-Nucleotidase, ecto |
| NUDT10 | GR508915 | 2 | NM_001035488 | 100% | 2e−138 | BT Nudix hydrolase 10 |
| NUDT11 | EG565272 | 3 | NM_001101296 | 100% | 0 | BT Nudix hydrolase 11 |
| P4HA3 | EG565269 | 2 | NM_001001598 | 100% | 1e−96 | BT Propyl 4-hydroxylase subunit alpha 3 |
| PAPSS2 | EG565283 | 1 | XM_583370 | 99% | 0 | BT 3′-Phosphoadenosine 5′-phosphosulfate synthase 2 |
| PIR | EG565279 | 1 | NM_001102358 | 100% | 1e−101 | BT Pirin (iron-binding nuclear protein) |
| PLA2G4A | EG565305 | 1 | AY363688 | 100% | 4e−164 | BT Phospholipase A2 group IVA |
| PLAT | EG565294 | 8 | NM_174146 | 99% | 0 | BT Plasminogen activator, tissue type |
| POSTN | EG565275 | 33 | AY445072 | 100% | 0 | BT Periostin |
| PSAP | EG565278 | 2 | NM_174161 | 99% | 8e−89 | BT Prosaposin |
| PTGES | EG565342 | 1 | AY032727 | 100% | 1e−122 | BT Prostaglandin E synthase |
| PTGS2 | EG565287 | 24 | AF031698 | 100% | 0 | BT Prostaglandin-endoperoxide synthase 2 |
| PTX3 | EG565317 | 2 | NM_001076259 | 98% | 3e−61 | BT Pentraxin |
| RBP1 | EG565267 | 1 | NM_001025343 | 99% | 5e−101 | BT Retinol binding protein 1 |
| RGS2 | EG565346 | 4 | NM_001075596 | 100% | 7e−130 | BT Regulator of G-protein signaling 2 |
| RND3 | EG565300 | 1 | NM_001099104 | 100% | 1e−127 | BT Rho family GTPase 3 |
| SAT1 | EG565349 | 26 | NM_001034333 | 99% | 0 | BT Spermidine/spermine N1-acetyltransferase 1 |
| SDC4 | EG565289 | 3 | XM_584869 | 99% | 1e−136 | BT Syndecan 4 |
| SEH1L | EG565340 | 1 | NM_001103089 | 99% | 1e−158 | BT SEH1-like nucleoporin |
| SLC25A17 | EG565266 | 1 | NM_001045948 | 99% | 0 | BT Solute carrier family 25 member 17 |
| SOX4 | EG565285 | 1 | NC_007324 | 99% | 8e−173 | BT SRY-box 4 |
| SULT1A1 | EG565308 | 1 | NM_177521 | 99% | 2e−99 | BT Sulfotransferase family 1A member 1 |
| SULT1E1 | GR508916 | 2 | NM_177488 | 99% | 4e−60 | BT Sulfotransferase family 1E member 1 |
| TIMP1 | GR508917 | 3 | NM_174471 | 100% | 2e−90 | BT TIMP metallopeptidase inhibitor 1 |
| TIMP2 | EG565302 | 1 | NM_174472 | 99% | 5e−163 | BT TIMP metallopeptidase inhibitor 2 |
| TMEM176B | EG565301 | 1 | NM_001099145 | 99% | 3e−165 | BT Transmembrane protein 176B |
| TMSB4X | EG565291 | 3 | NM_001113231 | 99% | 5e−173 | BT Thymosin beta 4, X-linked |
| TMSB10 | EG565264 | 1 | NM_174623 | 100% | 2e−131 | BT Thymosin beta 10 |
| TNC | EG565326 | 2 | NM_001078026 | 99% | 0 | BT Tenascin C |
| TNFAIP6 | EG565319 | 5 | NM_001007813 | 99% | 0 | BT Tumor necrosis factor alpha-induced protein 6 |
| USP53 | EG565304 | 1 | XM_015463685 | 100% | 8e−78 | BT Ubiquitin specific peptidase 53 |
| VNN2 | EG565337 | 2 | NM_001163920 | 100% | 0 | BT Vanin 2 |
| EST | EG565334 | 1 | AC_000158 | 100% | 3e−74 | BT transcribed locus, chromosome 1 |
| EST | EG565313 | 8 | AC_000159 | 99% | 3e−143 | BT transcribed locus, chromosome 2 [3’UTR of TNFAIP6] |
| EST | EG565321 | 4 | AC_000159 | 100% | 0 | BT transcribed locus, chromosome 2 [intron 1 of TNFAIP6] |
| EST | EG565339 | 2 | AC_000159 | 99% | 0 | BT transcribed locus, chromosome 2 [intron 1 of TNFAIP6] |
| EST | EG565316 | 1 | AC_000159 | 99% | 0 | BT transcribed locus, chromosome 2 [intron 1 of TNFAIP6] |
| EST | EG565341 | 2 | AC_000159 | 99% | 4e−70 | BT transcribed locus, chromosome 2 [3’UTR of TNFAIP6] |
| EST | EG565336 | 1 | AC_000161 | 99% | 0 | BT transcribed locus, chromosome 4 [3’UTR of Ubiquitin-conjugated enzyme E2H; UBE2H] |
| EST | EG565296 | 1 | AC_000163 | 100% | 0 | BT transcribed locus, chromosome 6 |
| EST | EG565307 | 1 | AC_000167 | 98% | 4e−106 | BT transcribed locus, chromosome 10 [intron 2 of Nidogen 2; NID2] |
| EST | EG565328 | 1 | AC_000169 | 99% | 0 | BT transcribed locus, chromosome 12 [intron 1 of Glypican-6; GPC6] |
| EST | EG565274 | 1 | AC_000169 | 99% | 3e−133 | BT transcribed locus, chromosome 12 |
| EST | EG565270 | 1 | AC_000172 | 99% | 8e−175 | BT transcribed locus, chromosome 15 [3’UTR of Rho GTPase activating protein 20; ARHGAP20] |
| EST | EG565314 | 1 | AC_000179 | 100% | 6e−156 | BT transcribed locus, chromosome 22 [intron 28 of ADAM metallopeptidase with thrombospondin type 1 motif 9; ADAMTS9] |
| EST | EG565295 | 1 | AC_000180 | 100% | 0 | BT transcribed locus, chromosome 23 [intron of runt related transcription factor 2; RUNX2] |
| EST | EG565309 | 1 | AC_000185 | 99% | 0 | BT transcribed locus, chromosome 28 [3’UTR of Sterile alpha motif domain containing 8; SAMD8] |
aGenBank accession numbers of differentially expressed bovine SSH cDNA clones
b Freq Frequency of cDNA clone identified from macroarray analyses of OF-DF subtracted library
cAccession number of the best match found following nucleotide sequence comparison via BLAST search in GenBank
dIdentity (%) represents homology estimates of bovine SSH cDNA fragments with nucleotide sequences in GenBank via BLAST search
e BT Bos taurus, EST expressed sequence tag, UTR untranslated region