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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Genesis. 2017 Sep 19;55(10):10.1002/dvg.23067. doi: 10.1002/dvg.23067

Figure 1. RC∷L-DTA allele.

Figure 1

(a) Schematic diagram of the Gt(ROSA)26Sor locus showing insertion of the CAG promoter, EGFP, and DTA cDNA. Open rectangles represent noncoding exons of Gt(ROSA)26Sor, and solid lines represent introns. DTA is oriented antisense to the CAG promoter and is located between two pairs of antiparallel lox sites (wildtype loxP and variant lox2272) which form the Cre-dependent FLEx Switch. WPRE, woodchuck hepatitis virus post-transcriptional regulatory element (Zufferey et al., 1999); poly(A), rabbit β-globin polyadenylation cassette (following EGFP) or bovine growth hormone polyadenylation cassette (following WPRE). (b) In the absence of Cre recombinase, ubiquitous EGFP expression is observed in RC∷L-DTA heterozygous embryos at E11.5. Image shows native fluorescence in a fixed embryo. Scale bar, 2 mm.