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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Allergy Clin Immunol. 2017 May 4;141(2):539–548.e7. doi: 10.1016/j.jaci.2017.04.016

Figure 5.

Figure 5

Validation of the subset of genes in the second cohort of samples by qRT-PCR. Log10 fold differential gene expression was compared between obese asthmatic and normal-weight asthmatic Th cells. In addition to genes investigated in the verification experiment (Figure 4), differential expression of additional genes downstream of CDC42 including MLK3 and cRAF1 were investigated. While expression of MLK3 was higher in obese asthmatic Th cells, that of cRAF1 did not differ between obese and normal-weight asthmatic Th cells.