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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Allergy Clin Immunol. 2017 May 4;141(2):659–670.e2. doi: 10.1016/j.jaci.2017.04.020

Figure 1.

Figure 1

Oral PE administration in pups prior to sensitization induces tolerance to peanut protein. (A) Protocol for induction of peanut allergy and induction of tolerance to peanut protein. PE was fed to pups from day −28 to day −15. Sensitization was performed on days 1, 7, and 21. Challenge was performed from day 35 to day 41. (B) Kinetics of the development of diarrhea after feeding PE prior to sensitization. (C) Scores based on the severity of clinical signs were assessed 30 minutes after oral challenge. (D) Serum levels of peanut-specific IgE, IgG1, and IgG2a were assessed by ELISA 24 hrs after the last challenge and expressed as optical density of diluted serum. Results were obtained from 3 individual experiments with 4 mice per group. ** P<0.01, # P<0.001, n.s. not significant. PBS/PBS/PE, sham-fed and sham-sensitized and peanut challenged; PBS/PE/PE, sham-fed and peanut sensitized and challenged; PE0.05/PE/PE, fed low doses of PE and PE sensitized and challenged; PE0.5/PE/PE, fed high doses of PE and PE sensitized and challenged.