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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Allergy Clin Immunol. 2017 May 4;141(2):659–670.e2. doi: 10.1016/j.jaci.2017.04.020

Figure 2.

Figure 2

Numbers of Tregs and FoxP3 expression in MLN CD4+ T cells and jejunum. (A) FoxP3 mRNA expression detected by quantitative RT-PCR in MLN CD4+ T cells from PBS/PBS/PE, PBS/PE/PE, PE0.05/PE/PE, and PE0.5/PE/PE mice. (B) Mucosal FoxP3-expressing cells were identified following immunohistochemical staining with anti-FoxP3 antibody. Representative sections of (a) PBS/PBS/PE mice, (b) PBS/PE/PE mice, (c) PE0.05/PE/PE mice, (d) PE0.5/PE/PE mice. (C) Quantitation of mucosal FoxP3-expressing cells. (D) Representative flow cytometric analysis of the percentage of FoxP3-expressing cells among the CD4+CD25+ T cells from MLN. Results were obtained from 3 individual experiments with 4 mice per group. Magnification x200. **P<0.01, n.s. not significant.