Fig. 1.

Heatmap for VP1 NT usage.

(A) Heatmap color shows the frequency of VP1 nucleotides from position 1 to position 903 (horizontal axis) in a multiple alignment. The vertical axis represents nucleotides and VPVD category (c or i). The scale is from 0 to 1.

(B) The frequency difference between cVDPV2 and iVDPV2 (scaled between − 0.7 and 0.7); red vertical lines represent usage of a particular nucleotide that is more utilized in iVDPV2 than cVDPV2 in a particular position, and vice versa for green lines.