Fig. 1.

Optimization of Indirect and Sandwich dot–blot assay. a Indirect dot–blot: Rabbit anti-SEA-polyclonal as detector antibody. b Indirect dot–blot: Mouse anti-SEA-monoclonal as detector antibody. c Sandwich dot–blot: Mouse anti-SEA-monoclonal as capture antibody and rabbit anti-SEA-polyclonal as detector antibody. d Sandwich dot–blot: Rabbit anti-SEA-polyclonal as capture antibody and mouse anti-SEA-monoclonal as detector antibody. (1) 1:1000 diluted detector antibody, (2) 1:2000 diluted detector antibody, (3) 1:4000 diluted detector antibody, (4) 1:8000 diluted detector antibody. (a) 1:25 diluted capture SEA antigen, (b) 1:125 diluted capture SEA antigen, (c) 1:625 diluted capture SEA antigen, (d) 1:3125 diluted capture SEA antigen, (e) negative control, (f) undiluted capture Antibody, (g) 1:5 diluted capture antibody, (h) 1:25 diluted capture antibody, (i) 1:125 diluted capture antibody, (j) 1:625 diluted capture antibody, (k) negative control