Table 1.
Incubation period (h) | Feed | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Glucose (1%, v/v) | Crude glycerol (1%, v/v) | Glucose (0.1%, w/v) + crude glycerol (1%, v/v) + propionic acid (0.5%, v/v) | |||||||||||||
DCM | PHB | Yield | PHV | Yield | DCM | PHB | Yield | PHV | Yield | DCM | PHB | Yield | PHV | Yield | |
(mg/L) | (%) | (mg/L) | (%) | (mg/L) | (%) | (mg/L) | (%) | (mg/L) | (%) | (mg/L) | (%) | ||||
Bacillus thuringiensis EGU45 | |||||||||||||||
24 | 640 | 75 | 12 | 0 | NA | 690 | 275 | 40 | 0 | NA | 960 | 150 | 16 | 5 | 0.5 |
48 | 580 | 70 | 12 | 0 | NA | 1880 | 1200 | 64 | 0 | NA | 890 | 155 | 17 | 70 | 8 |
72 | 600 | 65 | 11 | 0 | NA | 2390 | 1350 | 56 | 0 | NA | 2000 | 305 | 15 | 20 | 10 |
96 | 550 | 75 | 14 | 0 | NA | 2480 | 1480 | 60 | 20 | 0.8 | 2830 | 625 | 22 | 5 | 0.2 |
120 | 620 | 70 | 11 | 0 | NA | 3820 | 2725 | 71 | 20 | 0.5 | 4410 | 440 | 10 | 5 | 0.1 |
144 | 910 | 65 | 7 | 0 | NA | 3290 | 1450 | 44 | 6 | 0.2 | 4590 | 240 | 05 | 5 | 0.1 |
168 | 500 | 20 | 4 | 0 | NA | 3960 | 620 | 16 | 0 | NA | 4750 | 200 | 4 | 0 | NA |
192 | 450 | 15 | 3 | 0 | NA | 1510 | 450 | 30 | 0 | NA | 4900 | 150 | 3 | 0 | NA |
Bacillus cereus EGU43 | |||||||||||||||
24 | 490 | 25 | 5 | 0 | NA | 270 | 0 | 0 | 0 | NA | 1750 | 30 | 2.1 | 15 | 0.8 |
48 | 840 | 30 | 4 | 0 | NA | 360 | 100 | 28 | 0 | NA | 1460 | 50 | 3.4 | 170 | 12 |
72 | 2400 | 40 | 2 | 0 | NA | 830 | 405 | 49 | 0 | NA | 1800 | 80 | 4.4 | 100 | 6 |
96 | 3720 | 30 | 1 | 0 | NA | 940 | 355 | 38 | 0 | NA | 2220 | 130 | 5.8 | 125 | 6 |
120 | 3690 | 0 | 0 | 0 | NA | 1980 | 270 | 14 | 135 | 7 | 3130 | 65 | 2.1 | 290 | 9 |
144 | 4530 | 0 | 0 | 0 | NA | 3410 | 250 | 7 | 9 | 0.3 | 3190 | 165 | 5.2 | 0 | NA |
168 | 3500 | 0 | 0 | 0 | NA | 3430 | 1280 | 37 | 0 | NA | 3000 | 150 | 5 | 0 | NA |
192 | 3000 | 0 | 0 | 0 | NA | 2050 | 600 | 29 | 0 | NA | 2500 | 100 | 4 | 0 | NA |
Bacillus cereus EGU520 | |||||||||||||||
24 | 710 | 120 | 17 | 0 | NA | 1380 | 465 | 34 | 0 | NA | 700 | 0 | 0 | 0 | NA |
48 | 970 | 150 | 15 | 0 | NA | 1680 | 1090 | 65 | 0 | NA | 540 | 0 | 0 | 0 | NA |
72 | 2500 | 1000 | 40 | 0 | NA | 1900 | 905 | 47 | 0 | NA | 2000 | 20 | 1 | 0 | NA |
96 | 3540 | 2415 | 68 | 0 | NA | 2160 | 885 | 41 | 0 | NA | 2620 | 40 | 1.5 | 0 | NA |
120 | 1930 | 890 | 46 | 0 | NA | 2860 | 270 | 9 | 0 | NA | 3240 | 75 | 2.3 | 0 | NA |
144 | 1450 | 860 | 59 | 0 | NA | 2170 | 200 | 9 | 0 | NA | 4250 | 135 | 3 | 0 | NA |
168 | 1200 | 500 | 41 | 0 | NA | 1500 | 280 | 18 | 0 | NA | 4000 | 85 | 2.1 | 0 | NA |
192 | 1550 | 350 | 22 | 0 | NA | 1000 | 130 | 13 | 0 | NA | 3500 | 70 | 2.0 | 0 | NA |
DCM Dry cell mass PHB Polyhydroxybutyrate PHV Polyhydroxyvalerate NA Not applicable