Table 1.
Plasmids and bacterial strains used in this study
| Strains and plasmids | Phenotype | Source or references |
|---|---|---|
| E. coli strains | ||
| DH5α | SupE44ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 gyrA96 thi-1 relA1 | Sangon |
| MC1061 λpir | lacY1 galK2 ara-14 xyl-5 supE44 λpir | Rubires et al. [31] |
| MC1061-pRE112-toxR | MC1061 containing plasmid of pRE112-toxR, Cmr | This study |
| S17-1 λpir | Tpr Smr recA thi pro hsdR−M+RP4:2-Tc:Mu:Km Tn7 λpir | |
| S17-1-pRE112-toxR | S17-1 containing plasmid of pRE112-toxR, Cmr | This study |
| Vibiro alginolyticus strain | ||
| HY9901 | Wild-type, isolated from the diseased fish | Cai et al. [5] |
| HY9901-pRE112-toxR | HY9901 containing plasmid of pRE112-toxR, Cmr | This study |
| HY9901ΔtoxR | HY9901 carrying an in-frame deletion of toxR 206-812 | This study |
| C-HY9901ΔtoxR | HY9901ΔtoxR containing plasmid of pACYC184-toxR, Cmr | This study |
| Plasmid | ||
| pMD18-T | Cloning vector; Ampr | TaKaRa |
| pRE112 | pGP704 suicide plasmid, pir dependent, oriT, oriV, sacB, Cmr | Simon et al. [32] |
| pRE112-toxR | pRE112 containing toxR gene in-frame deletion of toxR 206-812, Cmr | This study |
| pACYC184 | Cmr, Tcr | Amersham |
| pACYC184-toxR | pACYC184 containing toxR gene, Cmr | This study |