Table 1.
Composition of different culture media used for callus induction, regeneration and root induction
| Medium name | PGRs (mg/l) | Medium name | PGRs (mg/l) |
|---|---|---|---|
| Callus Induction medium (CI) (full MS mediuma 34.14 g/l, agar 8 g/l, pH 5.8) | |||
| CI1 | Full MS without any PGRs | CI7 | Picloram (2.5) |
| CI2 | 2,4-D (1.5) | CI8 | Dicamba (1.5) |
| CI3 | 2,4-D (2.0) | CI9 | Dicamba (2.0) |
| CI4 | 2,4-D (2.5) | CI10 | Dicamba (2.5) |
| CI5 | Picloram (1.5) | CI11 | 2,4-D (4.0) + NAA (2.0) |
| CI6 | Picloram (2.0) | CI12 | 2,4-D (2.0) + Picloram (2.0) |
| Regeneration medium (RM) (full MS medium 34.14 g/l, agar 8 g/l, pH 5.8) | |||
| RM1 | Full MS without any PGRs | RM21 | BAP (1.5) + Kinetin (1.5) + IAA (0.5) |
| RM2 | BAP (1.5) | RM22 | BAP (1.0) + Kinetin (2.0) + IAA (0.5) |
| RM3 | BAP (2.0) | RM23 | BAP (0.5) + Kinetin (2.5) + IAA (0.5) |
| RM4 | BAP (2.5) | RM24 | 2,4-D (0.1) |
| RM5 | Kinetin (1.5) | RM25 | 2,4-D (0.1) + Zeatin (1.0) |
| RM6 | Kinetin (2.0) | RM26 | 2,4-D (0.1) + Zeatin (2.0) |
| RM7 | Kinetin (2.5) | RM27 | 2,4-D (0.1) + Zeatin (3.0) |
| RM8 | BAP (2.0) + IAA(0.1) | RM28 | 2,4-D (0.1) + Zeatin (4.0) |
| RM9 | BAP (2.0) + IAA(0.2) | RM29 | 2,4-D (0.1) + Zeatin (5.0) |
| RM10 | BAP (2.0) + IAA(0.3) | RM30 | 2,4-D (0.1) + CuSO4 (12) |
| RM11 | BAP (2.0) + IAA(0.4) | RM31 | 2,4-D (0.1) + CuSO4 (15) |
| RM12 | BAP (2.0) + IAA(0.5) | RM32 | 2,4-D (0.1) + CuSO4 (18) |
| RM13 | BAP (2.0) + 2,4-D(0.2) | RM33 | 2,4-D (0.1) + CuSO4 (21) |
| RM14 | BAP (2.5) + Kinetin (0.5) + IAA (0.1) | RM34 | 2,4-D (0.1) + CuSO4 (25) |
| RM15 | BAP (2.0) + Kinetin (1.0) + IAA (0.1) | RM35 | 2,4-D (0.1) + Zeatin (5.0) + CuSO4 (12) |
| RM16 | BAP (1.5) + Kinetin (1.5) + IAA (0.1) | RM36 | 2,4-D (0.1) + Zeatin (5.0) + CuSO4 (15) |
| RM17 | BAP (1.0) + Kinetin (2.0) + IAA (0.1) | RM37 | 2,4-D (0.1) + Zeatin (5.0) + CuSO4 (18) |
| RM18 | BAP (0.5) + Kinetin (2.5) + IAA (0.1) | RM38 | 2,4-D (0.1) + Zeatin (5.0) + CuSO4 (21) |
| RM19 | BAP (2.5) + Kinetin (0.5) + IAA (0.5) | RM39 | 2,4-D (0.1) + Zeatin (5.0) + CuSO4 (25) |
| RM20 | BAP (2.0) + Kinetin (1.0) + IAA (0.5) | ||
| Rooting medium (RTM) (half MS medium 17.21 g/l, agar 8 g/l, pH 5.8) | |||
| RTM1 | Half MS without any PGRs | RTM5 | NAA (0.2) |
| RTM2 | IAA (0.1) | RTM6 | IAA (0.1) + NAA (0.1) |
| RTM3 | IAA (0.2) | RTM7 | IAA (0.2) + NAA (0.2) |
| RTM4 | NAA (0.1) | ||
a MS medium composition and preparation (see supplementary Table S1)