Table 2.
Medium | Mature embryos | Immature embryos | ||||||
---|---|---|---|---|---|---|---|---|
Callus induction (%) | Precocious germination (%) | Callus size (cm) | Callus weight (mg) | Callus induction (%) | Precocious germination (%) | Callus size (cm) | Callus weight (mg) | |
CI1 | 0 | 100a | 0 | 0 | 0 | 100a | 0 | 0 |
CI2 | 97.96a | 2.04g | 0.41j | 38i | 100a | 0 | 0.50h | 42j |
CI3 | 96.79ab | 3.21fg | 0.50h | 43h | 99.67a | 0.33e | 0.50h | 43j |
CI4 | 95.00b | 5.00f | 0.52gh | 47g | 99.37a | 0.63e | 0.50h | 46i |
CI5 | 92.51c | 7.49e | 0.58f | 74e | 96.37b | 3.63d | 0.58f | 75f |
CI6 | 92.52c | 7.48e | 0.68e | 85d | 96.53b | 3.47d | 0.77e | 86e |
CI7 | 90.98cd | 9.03de | 0.73d | 87d | 95.64bc | 4.36cd | 0.80d | 87d |
CI8 | 87.71e | 12.29c | 0.82c | 108c | 92.26d | 7.75b | 0.90c | 114c |
CI9 | 85.76ef | 14.24bc | 0.97b | 120b | 91.54d | 8.46b | 1.00b | 124b |
CI10 | 85.26f | 14.74b | 1.07a | 127a | 92.67d | 7.33b | 1.10a | 136a |
CI11 | 92.21c | 7.79e | 0.45i | 50g | 96.27bc | 3.73cd | 0.47i | 50h |
CI12 | 89.96d | 10.04d | 0.53g | 54f | 94.53c | 5.47c | 0.57g | 53g |
Data of each trait represents the average values of six wheat genotypes (DBW 88, DBW 90, DBW 93, DPW 621-50, HD 3086 and WH 1105)
The figure followed by same superscript letters are not significantly different according to Tukey’s multiple comparison test at P < 0.01. CI6 gave the optimal callus induction