Table 2.
Details on the characteristics of the three field experiments used to assess effects of inoculation of beneficial soil organisms (pseudomonads, entomopathogenic nematodes, and arbuscular mycorrhizal fungi) on growth, health, and yield of spring wheat.
| Field trials | COMBINATION | PERFORMANCE-1 | PERFORMANCE-2 |
|---|---|---|---|
| Coordinates SN/EW | 46.397676/6.260763 | 46.397455/6.260166 | 46.39502/6.260444 |
| Sowing/inoculation day | 18th March 2014 | 18th March 2014 | 27th March 2015 |
| Treatments (Treatments codes refer to Table 1) |
Control (no inoculants) B1: P. protegens CHA0-Rif B2: P. chlororaphis PCL1391-Rif N1: H. megidis Andermatt N2: H. bacteriophora Andermatt N3: S. carpocapsae D83 N4: S. feltiae RS5 F1-H: R. irregulare INOQ Top (high dosage, 250 ml/row) F1-L: R. irregulare INOQ Top (low dosage, 50 ml/row) F2: R. irregulare SAF22 F3: F. mosseae SAF11 F4: C. claroideum SAF12 AMF control (substrate only) B1+ N2 B1+ F1 N2 + F1 B1+ N2 + F1 |
Control (no inoculants) B1: P. protegens CHA0-Rif B2: P. chlororaphis PCL1391-Rif N2: H. bacteriophora Andermatt BM: B1 + B2 B1 + N2 B2 + N2 BM + N2 |
Control (no inoculants) BM: B1 + B2 NM: N1 + N2 + N4 F1-L AMF control (substrate only) BM + NM NM + F1-L BM + F1-L BM + NM + F1-L |
| Number of treatments | 17 | 8 | 9 |
| Number of replicates (plots) per treatment | Four replicates in randomized complete block design | Four replicates in randomized complete block design | Nine replicates in randomized complete block design |
| Size of plots | 1.5 m2 | 6.75 m2 | 9 m2 |
| Number of plant rows per plot | 5 | 5 | 5 |
| Wheat seeds per m of row | ~80 | ~140 | ~140 |
| Bacterial inoculum (CFU in 400 ml H2O per meter of row) | B1: 1.19 × 109
B2: 1.21 × 109 |
B1: 1.42 × 109
B2: 3.37 × 109 |
B1: 4.875 × 108
B2: 8.25 × 108 |
| AMF inoculum | F1-H: 80 F1-L: 16 F2: 250 F3: 250 F4: 250 AMF control: 250 |
Not contributed | F1-L: 16 AMF control: 16 |
| Nematode inoculum | 50 infective juveniles/cm2/4 L | 50 infective juveniles/cm2/8 L | 50 infectice juveniles/cm2/8 L |
| Pest insect stress | Heavy natural infestation with Oscinella frit | Heavy natural infestation with Oscinella frit | No relevant Oscinella frit infestation |
| Soil type and texture | Sandy loam (clay, 25.5%; silt, 34.3%; sand; 40.2%) | Sandy loam (clay, 25.5%; silt, 34.3%; sand, 40.2%) | Loam (clay, 14.5%; silt, 26.8%; sand, 58.7%) |