Figure 4.

Regulation of mRNA and protein expression of Cav-1 by chronic treatment with fluoxetine requires epidermal growth factor receptor (EGFR) or Src activation in astrocytes. Cells were incubated for 2 weeks without any drug (0 μM fluoxetine, control) or with 0.1, 0.5, 1, 5, or 10 μM fluoxetine in the presence of AG1478, an inhibitor of EGFR or PP1, an inhibitor of Src. (A,C) Southern blots from representative experiments. Similar results were obtained from three independent experiments. Average mRNA expression was quantitated as ratio between Cav-1 and TBP, used as a house-keeping gene. (B,D) Immunoblots from a representative experiments. Similar results were obtained from three independent experiments. Average protein expression was quantified as ratio between Cav-1 and β-actin, used as a house-keeping gene. SEM values are indicated by vertical bars. *Indicates statistically significant (P < 0.05) difference from all other groups but not from each other.