Figure 5.

Regulation of mRNA and protein expression of Cav-1 by chronic treatment with fluoxetine requires ERK or AKT activation in astrocytes. Cells were incubated for 2 weeks without any drug (0 μM fluoxetine, control) or with 0.1, 0.5, 1, 5, or 10 μM fluoxetine in the presence of U0126, an inhibitor of MEK or LY294002, an inhibitor of PI3K. (A,C) Southern blots from representative experiments. Similar results were obtained from three independent experiments. Average mRNA expression was quantified as ratio between Cav-1 and TBP, used as a house-keeping gene. (B,D) Immunoblots from a representative experiments. Similar results were obtained from three independent experiments. Average protein expression was quantified as ratio between Cav-1 and β-actin, used as a house-keeping gene. SEM values are indicated by vertical bars. *Indicates statistically significant (P < 0.05) difference from all other groups but not from each other.