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. 2017 Oct 31;8:784. doi: 10.3389/fphar.2017.00784

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Copyright © 2017 Zhang, Shen, Mao, Wang, Wang, Huang, Hu, Shou and Wen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Typical histopathology of the rabbit tibia. Yellow bold arrow: bone trabeculae; orange arrow: osteoblasts; green arrow: osteocytes; blue arrow: vessels. (B) The columns represent the mean ± SE from five rabbits per group, ^∗P < 0.05. N.Ob: osteoblast number, T.Ar: trabecular bone area, B.pm: bone perimeter. (C) Masson’s trichrome staining of the tibia at 8 weeks after treatment with VCS-L, VCS-H, and VCS-icariin. (D) The columns represent the mean ± SE of the rate of new bone formation from the analysis of Masson’s trichrome staining. (E) Immunohistochemical staining for OC at 8 weeks after treatment with VCS-L, VCS-H, and VCS-icariin. The columns represent the mean ± SD from six rabbits per group, ^∗P < 0.05.