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. 2017 Oct 31;8:784. doi: 10.3389/fphar.2017.00784

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Copyright © 2017 Zhang, Shen, Mao, Wang, Wang, Huang, Hu, Shou and Wen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The proliferation capability of ROBs treated with vancomycin, ^∗P < 0.05. (B) ROBs treated with vancomycin and icariin, ^∗P < 0.05. (C) Cell cycle analysis was performed by flow cytometry using propidium iodide DNA staining of ROBs. (D) The percentages of ROBs in the cell cycle phases were shown in the percentage histogram. Each value represented mean ± SD of three independent experiments. ^∗P < 0.05, compared to the control ROBs, ^∗∗P < 0.05 compared to ROBs treated with vancomycin. (E) ALP level of ROBs treated with vancomycin, ^∗P < 0.05. (F) ALP level of ROBs treated with vancomycin and icariin, ^∗P < 0.05. (G) ALP positive staining of ROBs treated with vancomycin and icariin.