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. 2017 Oct 31;8:2108. doi: 10.3389/fmicb.2017.02108

Figure 1.

Figure 1

HO-1 expression in epithelial cells treated with CoPP or SnPP and infected with HSV. (A,B) Western blot analyses of HO-1 expression in Vero (left) and HeLa cells (right) after treatment with HO-1-modulating drugs and/or infection with HSV-2 at an MOI 1 for 16 h. (C,D) Flow cytometry analyses of HO-1 expression in Vero (left) and HeLa cells (right) after treatment with HO-1-modulating drugs and/or infection with HSV-2 at an MOI 1 (MFI, mean fluorescence intensity). (E,F) Virus plaque formation (PFU, plaque forming units) in Vero (left) and HeLa cells (right) treated with HO-1-modulating drugs and then infected with 150 PFU of HSV-2. Plaque forming units were determined at 18 and 36 h post-infection for Vero and HeLa cells, respectively. Data are means ± SEM of three independent experiments. Representative images are shown for western blots. Two-way ANOVA, and Tukey's multiple comparison test were used for statistical analyses (**p < 0.01, ***p < 0.001).