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Design of FAP-BKα and MG-TCarb. (A) Peptide layout and membrane topology of FAP-BKα construct. (B) Structure of MG-TCarb dye. Activation by FAP binding produces fluorescence with 633 nm excitation and 668 nm emission. (C) Voltage response of FAP-BKα in varying concentrations of Ca2+ compared to the same isoform lacking N-terminal HA/FAP tag. Quantification of V50 and Hill slope are shown below for each construct.
