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. 2017 Oct 31;11:337. doi: 10.3389/fncel.2017.00337

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Copyright © 2017 Pratt, Kuljis, Homanics, He, Kolodieznyi, Dudem, Hollywood, Barth and Bruchez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hemagglutinin (HA) localization corresponds to same brain regions as endogenous BKα; not all BKα in PCs show HA reactivity. Sagittal cryosections of p60 WT (A,B) and FAP-BKα homozygous (C,D) brains were co-stained for BKα (A,C) and HA (B,D). BKα shows notably strong expression in hippocampal formation (HF), cerebellum (Cer), substantia nigra (SN). Expression is also evident in Substantia innominata (SI) and globus pallidus (GP), which appear as a continuous region. (B) HA staining does not produce specific signal in WT. (D) FAP-BKα Hom mice show localization of HA signal to be similar to BKα. FAP-BKα heterozygous brain is not shown due to insufficient signal-to-noise in whole brain under immunostaining conditions. (E) Confocal sections of PCs show BKα expression in somata and dendrites in the PC and molecular layers, respectively. HA immunoreactivity is more strongly localized to soma than total BKα. White arrowheads point to example local maxima showing BKα and HA signal. (F) Quantification of total BKα fluorescence in PC dendrites (each point corresponds to a field of view from two to three animals of each genotype, normalized to granule cell layer fluorescence). (G) Total calculated cell fluorescence for PC somata, background calculated from granule cell layer. ^∗∗P ≤ 10^-3, ^∗∗∗∗P ≤ 10^-5 compared to WT.