Fig. 4.

Stimulation of TLR-expressing HEK-Blue cells by icodextrins and raw materials. HEK-Blue-2 (A) and HEK-Blue-4 (B) cells were stimulated by the addition of icodextrins (I-10.01, I-10.02, I-10.03 and I-11.12) and raw materials (M-10.05 and M-10.07), all samples at the finale concentration of 3.75% (w/v). After 16 h of incubation, production of SEAP was quantified by measuring the phosphatase activity released in cell-free supernatants. Data are expressed as means values ± SEM from three separate experiments (*P < 0.05, significantly different by comparison with non-stimulated cells cultured in the absence of glucose polymer sample).