Fig. 5.

Effect of mutanolysin treatment on the inflammatory activity of PGN. Icodextrins and raw materials (37.5%, w/v in sterile PBS) were pre-treated in the absence or presence of mutanolysin (2500 U/mL) for 16 h at 37 °C. Samples were then added to HEK-Blue-2 cells (A), HEK-Blue-4 cells (B) or THP1 cells (C) (dilution 1/10). After 16 h of stimulation, the production of SEAP from HEK-Blue-2 and HEK-Blue-4 cells was quantified by measuring the phosphatase activity released in the supernatants. The production of RANTES from THP1 cells was measured in cell-free supernatants by ELISA. Data are expressed as means values ± SEM from three separate experiments (*P < 0.05, significantly different by comparison with non-stimulated cells cultured in the absence of glucose polymer sample).