Figure 2.

Timeline of the experiments. Neurons were differentiated in neurosphere culture for 8–10 weeks before adherent culture in tunnel devices on MEAs and in standard cell culture wells (control cells). MEA measurements were performed twice weekly over 5 weeks, and a pharmacological test was performed at the end of the culture. Neurite orientation analysis was performed from the phase contrast images taken at the 5th week. Immunocytochemistry was performed on control cells after 2 weeks in adherent culture for both cell lines.