FIGURE 4.

Insertion of the R6 domain in the promoter of MYB10 orthologs leads to autoregulation. (A) Schematic of the engineered pear MYB10 and AtMYB75 promoters fused to the LUC reporter gene in pGreen0800LUC. (B) Dual luciferase promoter assay of the R6 engineered promoters in N. benthamiana. Promoters, with or without the R6 domain, were co-infiltrated with MdMYB10 and MdbHLH3, PcMYB10 and AtbHLH2, or AtMYB75 and AtbHLH2. Luminescence of LUC and REN was measured 3 days post-infiltration and expressed as a ratio of LUC to REN. Data represent means (± SE) of four technical replicate reactions.