FIGURE 4.

LASSBio-897 decreases lung inflammation and fibrosis in silicotic mice. Silica (10 mg) was given i.n. and analyses were performed 28 days later. Oral treatment with LASSBio-897 was performed daily for 7 days, starting 21 days after silica instillation. Histological sections of mouse lungs provoked with silica (B,F) and treated with LASSBio-897 (5 mg/kg) (C,G). Animals instilled with PBS were used as controls (A,E). (D) Morphometric analyses of granuloma. (H) Quantitative analysis of collagen content in the lung tissue was performed by Sircol technique. Silicotic non-treated animals received an equal amount of vehicle (DMSO 0.1%). Data are expressed as the means ± SD from six mice. Scale bars = 200 μm. These results are representative of two independent assays. ⁺P < 0.05 compared to saline-provoked group. ^∗P < 0.05 compared to silica-provoked group.