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. 2017 Oct 31;8:778. doi: 10.3389/fphar.2017.00778

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Copyright © 2017 Carvalho, Ferreira, de Arantes, Noël, Tesch, Sant’Anna, Barreiro, Fraga, Rodrigues e Silva and Martins.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LASSBio-897 enhances activation of A_2A receptors evoked by adenosine in HEK293G cells. (A) HEK293G cells were concomitantly stimulated with LASSBio-897 and adenosine. HEK293G cells were treated with an A_2A receptor antagonist (B), or A_2B receptor antagonist (C) 30 min before the simultaneous addition of LASSBio-897 and adenosine. Immediately after stimulation, the kinetic traces for cAMP generation were measured by a luminometer. Control group received an equal amount of vehicle (DMSO 0.1%). Data points are means ± SD of triplicate determinations from a single experiment and are representative of three experiments. SCH, SCH 58261. PSB, PSB 603.