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. 2017 Oct 31;8:1344. doi: 10.3389/fimmu.2017.01344

Figure 4.

Figure 4

T-bet deficiency protects against the development of colitis and chronic lung inflammation induced by cigarette smoke (CS) exposure. T-bet−/+ and T-bet−/− mice were exposed to CS for 4 weeks. (A) Colon length was measured 24 h after last CS exposure, and (B) the body weight was monitored for 4 weeks. (C,D) The concentration of IFN-γ and TNF-α was measured in ascending colon tissue homogenate using Th1, 2, and 17 cytometric bead array. On day 27, the mice were sacrificed, and bronchoalveolar lavage (BAL) fluid was collected. (E) The total cells, neutrophils, macrophages, and lymphocytes in the BAL fluid from the lung of mice were counted using Diff-Quick staining. (F) The concentrations of the Th1 cytokines IFN-γ and TNF-α in the BAL fluid were determined using enzyme-linked immunosorbent assay. Data are shown as the mean ± SEM, and p value was estimated by unpaired t test (***p < 0.001, **p < 0.01, and *p < 0.05, ###p < 0.001, ##p < 0.01, and #p < 0.05, and not significant; ns).