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. 2017 Oct 31;8:1344. doi: 10.3389/fimmu.2017.01344

Figure 6.

Figure 6

IFN-γ plays a substantial role in colitis induced by lung draining lymph node CD4+CD25− T cells adoptive transfer (AT) in IL-10−/− mice. WT and IFN-γ−/− mice were exposed to cigarette smoke for 4 weeks. On day 27, CD4+CD25− T cells were isolated from lung draining lymph node cells of mice and were adoptively transferred via i.v. into IL-10−/− mice. On day 14 after AT, the mice were sacrificed. (A) Experimental design of colitis induction in IL-10−/− mice. The schematic illustration was generated by modifying images purchased in the PPT Drawing Toolkits-BIOLOGY Bundle (Motifolio Inc., Elliocott City, MD, USA). (B) Representative images of whole colons (each group) and colon length. (C) Body weight changes after of CD4+CD25− T cell AT. (D) Th1 cytokines IFN-γ and TNF-α mRNA expression in the colon was quantified by real-time PCR. (E) Representative images of whole colons (each group) and colon length. (F) Body weight changes after CD4+CD25− T cell AT. (G) Th1 cytokines IFN-γ and TNF-α mRNA expression in the colon was quantified by real-time PCR. Data are shown as the mean ± SEM, and p value was estimated by unpaired t test (***p < 0.001, **p < 0.01, and *p < 0.05 and not significant; ns).