Table 2.
Median staining intensity (score) of renal PKCβI, PKCβII, NHE1, and NHE3 protein localization.
| Median staining intensity (score) | ||||||||
|---|---|---|---|---|---|---|---|---|
| PKCβI | PKCβII | NHE1 | NHE3 | |||||
| Sham | Aldo | Sham | Aldo | Sham | Aldo | Sham | Aldo | |
| Cortex | ||||||||
| Glomerulus | 2 | 3 | 3 | 3 | 3 | 3 | 1 | 2 |
| PCT | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 1 |
| DCT | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 |
| CCD | 0 | 0 | 0 | 0 | 3 | 4 | 1 | 2 |
| Pcap | 2 | 3 | 3 | 3 | 2 | 2 | 1 | 1 |
|
| ||||||||
| Outer medulla | ||||||||
| TALH | 1 | 1 | 2 | 1 | 1 | 1 | 2 | 1 |
| MCD | 1 | 1 | 3 | 1 | 3 | 4 | 2 | 1 |
| VR | 3 | 4 | 3 | 3 | 1 | 4 | 2 | 4 |
| tLH | 3 | 4 | 2 | 3 | 1 | 4 | 2 | 4 |
|
| ||||||||
| Inner medulla | ||||||||
| MCD | 1 | 1 | 3 | 1 | 3 | 4 | 1 | 0 |
| VR | 1 | 2 | 1 | 1 | 1 | 2 | 1 | 4 |
| tLH | 1 | 2 | 2 | 1 | 1 | 2 | 1 | 2 |
Staining intensity: 0 = negative, no reactivity; 1 = trace, faint or pale brown staining with less membrane reactivity; 2 = weak, light brown staining with incomplete membrane reactivity; 3 = moderate, shaded of brown staining of intermediate darkness with usually almost complete membrane reactivity; 4 = strong, dark brown to black staining with usually complete membrane pattern, producing a thick outline of the cell [11–13]. PCT: proximal convoluted tubule; DCT: distal convoluted tubule; CCD: cortical collecting duct; Pcap: peritubular capillary; TALH: thick ascending limb of the loop of Henle; MCD: medullary collecting duct; VR: vasa recta; tLH: thin limb of the loop of Henle (n = 5/group).