Figure 4.

Kinetic profiles of insulin secretion from perifused EndoC-βH1 β-cell pseudoislets in response to a 30 min stimulation with leucine (20 mM) in the presence of glutamine (2 mM) (a) (n = 4) as well as with KCl (40 mM) (b) (n = 4). After a 20 min preperifusion phase with basal glucose (3 mM), a 30 min stimulation period and thereafter a return of the perifusion to basal glucose medium for another 30 min are depicted. Shown are means ± SEM of insulin release rates expressed as ng/min and per 100 pseudoislets (PI).